Institutioner vid LTH - Forskningsoutput - Lunds universitet

Faculty of Engineering, LTH - Research Outputs - Lund

Cyclodextrin glucanotransferase (CGTase) from Bacillus macerans was applied to produce the CDs from linear α- (1,4)-glucans, which were obtained by Neisseria polysaccharea amylosucrase (Np AS) treatment on sucrose. The greatest CD yield (21.1%, w/w) was achieved from a one-pot dual enzyme reaction at 40 °C for 24 h. Amyloglucosidase from Aspergillus niger (Spirizyme Fuel) and CGTase from Thermoanaerobacter sp. (Toruzyme 3.0 L) were kindly donated by Novozymes A/S. CGTase from Bacillus macerans (CGTase Amano) was kindly supplied by Amano Enzyme Inc. Pure amyloglucosidase from Aspergillus niger (ref.

Cgtase amano

(Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with increasing concentration, indicating the formation of the so‐called analogous series, Figure 2 The distance of CGTase (EC 2.4.1.19) from Bacillus macerans was purchased from the needle tip from the substrate was varied in the range of 10 Amano Enzyme, Inc. (Japan), and was used without further puriﬁ- to 25 cm. The electrospraying modes were observed using a digi- cation. The crude CGTase extract with the corn starch substrate showed a productivity of 0.38 mmol/L/h, which was 29 % lower than using the purified enzyme and the corn starch substrate but 7 % higher (Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with We are grateful for the kind gift of the CGTase enzyme isolated from Bacillus macerans provided by Amano Enzyme Inc., Nagoya, Japan.

(CGTase). Feruloil Esterasa. cyclomaltodextrin glycosyltransferase; konchizaimu; α-1,4-glucan 4- glycosyltransferase, cyclizing; BMA; CGTase; neutral-cyclodextrin glycosyltransferase; 31 Jul 1998 Ciclodextrina glucosiltransferase (CGTase E. C. 2.4.1.19) de Bacillus macerans da Amano International Enzyme Co. Inc. (Nagoya, Japão), com recubiertos con PEI de distinto tamaño………………………….

Faculty of Engineering, LTH - Research Outputs - Lund

Glucoamylase was from Toyobo Co., Ltd. (Osaka, Japan) and B. macerans CGTase was from Amano Enzyme Inc. (Aichi, Japan). The enzyme was further purified using DEAE Sepharose Fast Flow media (Amersham Biosciences Europe GmbH).

Faculty of Engineering, LTH - Research Outputs - Lund

2.2. the Synthesis of β-maltosides of resveratrol. CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) or resveratrol 4′-β-glucoside (3) (0.1mmol) and (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano).

Since 1951 Amano has been providing environmental solutions that improve the workplace. With a product offering which includes oil and water soluble mist col Umas das valorizações possíveis, com alto valor agregado, é a bioconversão enzimática da fécula de mandioca em Ciclodextrinas (CDs). Os primeiros trabalhos sobre este tema desenvolveram o esquema de produção das ciclodextrinas à partir da fécula de mandioca pela Cyclodextrin Glucanotransferase (CGTase) Amano. Î ² CGTase º ¢ Amano Pharmaceutical Co. ¦ V Bacillus macerans V · j Û Ö B ç Î ² (Lot No., CGRRU11529L, > w : pH 6, N ê 60 o C)¢ Ò Ï ~ & b , & ò B ~ α-, β-, Os primeiros trabalhos sobre este tema desenvolveram o esquema de produção das ciclodextrinas à partir da fécula de mandioca pela Cyclodextrin Glucanotransferase (CGTase) Amano.
Svedia

CGTase (Paenibacillus macerans) was purchased from Amano Enzyme Inc. Resveratrol 3-maltoside (8) and resveratrol 4′-β-maltoside (9) were produced individually as fol-lows. To a solution containing resveratrol 3-β-glucoside (2) or resveratrol 4′-β-glucoside (3) (0.1mmol) and (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such The enzyme from Thermoanaerobacter sp. (Toruzyme 3.0L) worked better than that from Bacillus macerans (CGTase Amano). The transglycosylation activity of CGTase is well reported, as it is able to glucosylate other phenolic compounds such as resveratrol [ 26, 27 ], catechin [ 28 ], hydroquinone [ 29 ], kaempferol [ 30] or genistein [ 31 ]. Table 1.

CGTase solution (1% v/v) and PVA (8wt%) solution were mixed followed by electrospinning (−9kV, 3h). CGTase/PVA nanofibres with an average diameter of 176±46nm were successfully produced. The nanofibres that consist of immobilised CGTase were crosslinked with glutaraldehyde vapour. The synthesis of curcumin oligosaccharides was performed by incubating the reaction mixture (10 mL) containing 0.2 mmol of curcumin d-glucoside, 5 g of soluble starch, and 200 units of CGTase from Bacillus macerans purchased from Amano Pharmaceutical Co. Ltd. in 25 mM sodium phosphate buffer (pH 7.0) at 40 °C for 24 hours. CGTase derived from Bacillus macerans was obtained as a kind gift from Amano Enzyme, Inc., Nagoya, Japan, in a stock solution that was used as supplied and stored at 5 °C.
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CGTase could be successfully immobilised into a nanofibrous polyvinyl alcohol (PVA) membrane (Saallah et al., 2016) by co-electrospinning of CGTase and PVA mixture, which allow the enzyme to attach andencapsulatedinthenanofibers.ThePVAsolution is rich with hydroxyl groups that enable the formation of secondary bonding with the enzyme molecules. 1996-01-01 · CGTase, which produces mainly ~-CD, seems to exist widely. The number of ~'-CD producing enzyme is increasing, partly because of ~'-CD's high solubility, its large cavity and its potential usefulness. In all of these CGTase producing bacteria, B. macerans was selected for our research, and cultivated to obtain the enzyme containing broth.

In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with We are grateful for the kind gift of the CGTase enzyme isolated from Bacillus macerans provided by Amano Enzyme Inc., Nagoya, Japan. We thank the Villum Foundation and Carlsberg Foundation for financial support. 7KLV (OHFWURQLF6XSSOHPHQWDU\0DWHULDO (6, IRU&KHP&RPP MRXUQDOLV 7KH 5R\DO 6RFLHW\RI&KHPLVWU\ Since 1951 Amano has been providing environmental solutions that improve the workplace. With a product offering which includes oil and water soluble mist col Î ² CGTase º ¢ Amano Pharmaceutical Co. ¦ V Bacillus macerans V · j Û Ö B ç Î ² (Lot No., CGRRU11529L, > w : pH 6, N ê 60 o C)¢ Ò Ï ~ & b , & ò B ~ α-, β-, Microbial production of neryl-α-D-glucopyranoside from nerol byAgrobacterium sp. M-12 reﬂects glucosyl transfer activity Kazuki Takahashi a, Issei Terauchi , Marie Ono a, Hiroshi Satohb and Makoto Ueda aOyama National College of Technology, Oyama, Japan; bNissei Bio Co., Eniwa City, Hokkaido, Japan ABSTRACT Terpene alcohol is widely used in perfumes and is known to possess antibacterial CGTase have focused on trying to understand the mechanism of the cyclization reaction and to find or engineer a CGTase that produces a specific type of CD. CGTases from B. macerans was purchased from Amano Pharma-ceutical Co., Ltd. (Aichi, Japan) and was used without further purifi-cation. Glucoamylase was from Toyobo Co., Ltd. (Osaka, Japan) and B. macerans CGTase was from Amano Enzyme Inc. (Aichi, Japan).
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Institutioner vid LTH - Forskningsoutput - Lunds universitet

7KLV (OHFWURQLF6XSSOHPHQWDU\0DWHULDO (6, IRU&KHP&RPP MRXUQDOLV 7KH 5R\DO 6RFLHW\RI&KHPLVWU\ Since 1951 Amano has been providing environmental solutions that improve the workplace. With a product offering which includes oil and water soluble mist col Î ² CGTase º ¢ Amano Pharmaceutical Co. ¦ V Bacillus macerans V · j Û Ö B ç Î ² (Lot No., CGRRU11529L, > w : pH 6, N ê 60 o C)¢ Ò Ï ~ & b , & ò B ~ α-, β-, Microbial production of neryl-α-D-glucopyranoside from nerol byAgrobacterium sp. M-12 reﬂects glucosyl transfer activity Kazuki Takahashi a, Issei Terauchi , Marie Ono a, Hiroshi Satohb and Makoto Ueda aOyama National College of Technology, Oyama, Japan; bNissei Bio Co., Eniwa City, Hokkaido, Japan ABSTRACT Terpene alcohol is widely used in perfumes and is known to possess antibacterial CGTase have focused on trying to understand the mechanism of the cyclization reaction and to find or engineer a CGTase that produces a specific type of CD. CGTases from B. macerans was purchased from Amano Pharma-ceutical Co., Ltd. (Aichi, Japan) and was used without further purifi-cation. Glucoamylase was from Toyobo Co., Ltd. (Osaka, Japan) and B. macerans CGTase was from Amano Enzyme Inc. (Aichi, Japan).